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  • A) Determination of cell proliferation by MTT assay: The colorimetric 3-(4, 5-dimethyle thiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay (Sigma) was used as previously described [38] to assess the growth stimulating effects of chronic epinephrine exposure and potential inhibitory effects of celecoxib on this response in Panc-1 and BXPC-3 cells in vitro.
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