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Culture of Cancer Cell Lines: The authenticated (RADIL, Columbia, MO), human pancreatic cancer cell lines BXPC-3 (witout ras mutations) and Panc-1 (expresses activating point mutations in K-ras) originally purchased from the American Type Culture Collection (Rockville, MD) were grown in RPMI-1640 with 10% fetal bovine serum without antibiotics.
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