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  • Final sequences for these phylotypes were deposited in GenBank with the accession numbers JQ581680-JQ582008 Characterization of 16S rRNA sequences for Gamma3 and Gamma4 phylotypes: To further characterize the novel clusters (Gamma3 and Gamma4), ∼50 ng of DNA from individual bee preps (AZ107.4, AZ109.1, and AZ109.3) was used to perform PCR amplification of near full-length 16S rRNA sequences, using the following universal primers: 27F′-HT (5′-AGRGTTTGATYMTGGCTCAG-3′) and 1492R′-HT (5′-GGYTACCTTGTTACGACTT-3′) [30].
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