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  • The Snodgrassella primer (betacd1 5′- TTCGCTACCCTCTGTACCGACCATT - 3′) or Gilliamella primer (gma1cd2 5′-TCGCCTCCCTTTGTATACGCCATT-3′) were paired with 16S rRNA universal primer 27Fshort (5′-GAGTTTGATCCTGGCTCA-3′) in 25 µL reactions of 0.8 U Taq DNA Polymerase (New England BioLabs), 0.25 mM of each dNTP, 1x PCR buffer, and 0.5 µM concentrations of each primer.
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