nif:isString
|
-
The forward primer was 926F454 Tit F 5′- CCTATCCCCTGTGTGCCTTGGCAGTCTCAG-aaactYaaaKgaattgacgg-3′ (Lib B adapter is in caps) and the reverse barcoded primer was 1392R454 Tit R 5′- CCATCTCATCCCTGCGTGTCTCCGACTCAG-NNNNN-acgggcggtgtgtRc (Lib A is in caps and the variable barcode region is denoted by N's).
|