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  • For postnatal acute antidepressant treatments, age-matched litters (P5-21) were randomly assigned to receive i.p. injection of saline (SAL) (NaCl 0.9%, 5 ml/kg), imipramine (IMI) (HCl salt, dissolved in SAL, 30 mg/kg, 5 ml/kg; Sigma-Aldrich Finland Oy, Helsinki), or clomipramine (HCl salt, dissolved in SAL, 20 mg/kg, 5 ml/kg; Sigma-Aldrich) and following indicated lag-time (30–60 min) hippocampus and medial prefrontal cortex were collected as described in [9].
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