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  • Membranes were incubated with the following primary antibodies: anti-p-TrkBY816 (1∶5000; kind gift from Dr. M. Chao, Skirball Institute, NY, USA), anti-p-TrkA/BY490/Y515 (#9141; 1∶2000; Cell Signalling Technology (CST), MA, USA), anti-p-TrkA/BY674-5/Y705-6 (1∶1000; CST), anti-TrkBout (#610102; 1∶2000; RD Transduction Laboratories, Franklin Lakes, NJ USA), anti-p-CREBS133 (#9198; 1∶1000; CST), anti-CREB (sc-186; 1∶100, Santa Cruz Biotechnology (SCB), CA, USA), anti-p-AktThr308 (#9275; 1∶2000, CST), anti-AKT (#4691; 1∶1000, CST), anti-p-PLCγ1Y783 (#2821, 1∶1000, CST) or anti-Trk (#sc-11 (rabbit), 1∶1000, SCB).
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