Phosphatase activity was determined by incubating 2 µL sample with 0.15 mM RII substrate (BIOMOL) and 5 nM tautomycin (to inhibit PP1) or 5 nM tautomycin + OA (to inhibit PP1 and PP2A activity) in 50 mM Tris-HCl, pH 7.0, 100 µM Na2EDTA, 5 mM DTT, 0.01% Brij35 at 30°C for 10 min.
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