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  • Whole hippocampi were dissected and homogenized in 3.75 mM Tris-HCl, pH 7.4, 15 mM KCl, 3.75 mM NaCl, 250 µM EDTA, 50 µM EGTA, 30% (w/v) sucrose, 30% (v/v) glycerol, protease inhibitor cocktail (Sigma), 100 µM PMSF using a Dounce homogenizer, then centrifuged (1000 g, 10 min).
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