The membrane was then rinsed and baked in a 80°C oven under a vacuum for 2 hr, rinsed 2 times in 2X SSC buffer (for 1 L 20X SSC: 3 M NaCl, 0.3 M Sodium Citrate, pH 7.0), and prehybridized in ULTRAHTb (Ambion) for 1 hr at 42°C in the hybridization chamber.
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