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  • Peptides were eluted from a 10 cm column (Picofrit 75 µm ID, New Objectives) packed in-house with ReproSil-Pur C18-AQ 3 µm reversed phase resin (Dr. Maisch, Ammerbuch Germany) using a 90 min acetonitrile/0.1% formic acid gradient at a flow rate of 200 nl/min to yield ∼15 s peak widths.
rdf:type