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  • The experimental animals were assigned to the following groups: (i) animals having stimulation in the reuniens nucleus and fEPSP recordings in the prefrontal cortex (n = 20); these animals were classically conditioned with a trace paradigm in the presence (n = 10) or absence (n = 10) of two HFS sessions; (ii) animals stimulated in the reuniens nucleus and with fEPSP recordings carried in the hippocampal CA1 layer (n = 20); half of these animals were presented with two HFS sessions before the first two conditioning sessions; (iii) mice (n = 20) presented with an object discrimination test in the presence (n = 10) or absence (n = 10) of an HFS stimulation session; (iv) animals (n = 10) presented with two HFS sessions, and the fEPSPs evoked by pairs of pulses (40 ms of interval) in the PFC and in the CA1 were followed for up to 3 d; and (v) ten additional animals used for the histological study.
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