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  • Plasmids and PCR analysis of transcripts: The CG11699 cDNA clone (RE61805) of 450 bp was obtained from the Drosophila Genomics Resource Center (Indiana University, Bloomington, IN) and amplified with primers harboring restriction sites at their 5′ ends (5′-AGACACTAGTATGAGCGAGGCCGGCACC-3′ carrying SpeI and 5′-ACTAGCGGCCGCCTTCCTTGTTCCAGGC-3′ carrying NotI) using Isis Taq DNA polymerase (Qbiogene).
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