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  • Endogenous peroxidase activity was blocked by incubating the sections for 20 min in 0.6% hydrogen peroxide, the non-specific antibody binding sites were blocked with 5% goat normal serum, and the sections were incubated with the primary antibody at 4°C (rabbit anti-quail recombinant ARO antibody, QR2/05 (gift from Prof. N. Harada, Fujita Health University, Toyoake, Japan) 1∶3000, overnight incubation) or rabbit anti-VT antibody (gift from Dr. D.G. Gray, Max Plank Institute of Bad Nauheim, Germany) 1∶5000, 48 hours incubation).
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