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  • New aPKC constructs: we mutated two consensus GSK3 (Ser330 and Thr422 according to the Drosophila aPKC coding sequence) sites in aPKC to alanines using double strand mutagenesis (QuickChange, Stratagene), and cloned this otherwise full-length aPKC construct into pUASt vector using Gateway technology (Invitrogen).
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