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  • In brief, immediately after retinal dissection, retinas were permeabilized in PBS 0.1 M (pH 7.2-7.4) 0.5% Triton® X-100 by freezing them overnight at −70 °C, rinsed in new PBS 0.5% Triton®X-100, and incubated the following day at 4 °C with goat anti-Brn3a (C-20) antibody (Santa Cruz Biotechnologies Heidelberg, Germany) diluted 1:100 in blocking buffer (PBS, 2% BSA (BAS), 2% Triton®X-100).
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