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  • Both retinas were dissected and prepared as flattened whole-mounts by making four radial cuts (the deepest in the superior pole); post-fixed for an additional hour; rinsed in 0.1 M PB; mounted vitreal side up on subbed slides; and covered with antifading mounting media containing 50% glycerol and 0.04% p-phenylenediamine in 0.1 M sodium carbonate buffer (pH 9).
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