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  • To identify RGCs that retain their axoplasmic transport after lasering, hydroxystilbamidine methanesulfonate (OHSt) (Molecular Probes, Leiden, The Netherlands), a small molecule (472.53 kDa Mw) with similar fluorescent and tracer properties to Fluorogold® [67], was applied to both superior colliculi (SCi) one week before animal sacrifice following previously described methods that are standard in our laboratory [51,68].
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