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  • DNA was amplified in a reaction comprised of: 20 ng genomic DNA, 1× buffer I (1.5 mM MgCl2, Applied Biosystems, Foster City, CA), 1 mM dNTPs (Applied Biosystems), 0.4 µM primer (each of forward and reverse, IDT, Coralville, IA), and 0.25 units AmpliTaq Gold (Applied Biosystems) in a total volume of 10 µl.
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