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  • The channel current was recorded using a patch-clamp amplifier (Axon 200B; Axon Instruments, http://www.axon.com), low-pass-filtered at 1 kHz (−3 dB) by an eight-pole Bessel filter, digitized by an AD converter (Digidata; Axon Instruments), and continuously acquired on a computer (Dell) with commercially available software (pCLAMP9; Axon Instruments).
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