nif:isString
|
-
Second, pileup files were generated for each isolate based on the above-mentioned list to determine the appropriate nucleotide state for positions in the list for each isolate based on the following rules: a) if there was no mapped reads at a position it was treated as missing data; b) if different nucleotides were called at the position, the one with frequency larger than 50% was the consensus call for that position; and c) if different nucleotides were called at a position but none had a frequency larger than 50%, that position for that individual isolate was coded as missing data.
|