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  • Bundles of 16 microwire electrodes (platinum-iridium, formvar coated, 25-μm diameter, with an impedance of 250–500 kΩ measured at 1 kHz; California Fine Wire, Grover Beach, CA), along with one 50-μm diameter reference electrode made of the same material and insulation (California Fine Wire, Grover Beach, CA) were lowered into the guide cannula and protruded 1 mm below the distal end of the guide cannula (Fig 1).
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