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  • Following electrophoresis gels were transferred by semi dry transblot (Biorad) to nitrocellulose membranes and probed against rabbit polyclonal AKT, pAKT, mouse monoclonal ERK, pERK and rabbit monoclonal GSK3ß, pGSK3ß, CREB and pCREB antibody (Sigma, St. Louis, Mo., USA) at a concentration 1∶1000, and further with respective secondary antibody labelled with horseradish-peroxidase at a concentration 1∶5000 (Santa Cruz, CA).
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