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  • Cells were seeded in 6-well plates at a density of 0.3×106 cells per plate in Dulbecco’s modified Eagle’s medium (DMEM) (Life Technologies, Burlington, ON) supplemented with 10% fetal bovine serum (FBS) (Life Technologies), 2 mM L-glutamine (Life Technologies), and 100 mg/mL penicillin-streptomycin and grown to confluency at 37°C in 5% CO2.
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